Journal of clinical immunoassay Juan-Ping Yu, Institute of Antibody Engineering, School of Biotechnology, Southern Medical University, Guangzhou, P. When evaluated with clinical nasopharyngeal swabs, the assay was performed at >80% sensitivity, >84% specificity, and > 82% accuracy for detection of the S1-RBD antigen. }, author={Guifeng Yan and Huangxian Ju ImmoAnalysis as the second journal in this field will provide a platform for the publication of studies on all scientific aspects of immune analysis, including developing bioanalytical methods such as quantification of small molecules, peptides, proteins, antibodies, biomarkers, and immunoassay methods, and studies in immunology field. The merits of the procedure versus those of nonparametric methods of estimating the dilution inducing a 50% response rate are discussed. Groot C, Cicognola C, Bali D, et al. Diagnostic and prognostic performance to detect Alzheimer’s disease and clinical progression of a novel assay for plasma P-Tau217. Open Access. pallidum IgG chemiluminescence immunoassay (CLIA) and the T. 9 and 1. 9 at 13. We evaluated Here, we developed a rapid, simple, and sensitive immunoassay based on the split-luciferase system to detect IgG antibodies against African swine fever virus (ASFV). Journal Search Engine. Sometimes, these results can mislead or miss a diagnosis resulting in unnecessary investigations and mental trauma to the patient and families. Serum and plasma calprotectin concentration is shown to be elevated when neutrophils are activated, and may therefore be used as a marker for inflammatory diseases. Air Pollution and Breast Cancer Incidence Dr. A fully automated, novel high-sensitivity hepatitis B core-related antigen assay (iTACT-HBcrAg) has been developed. Treatment was blinded to assay results. Check Out IF Ranking, Prediction, Trend & Key Factor Analysis. ChIVD LiCA anti-HCV assay was a highly Light‐initiated chemiluminescent assay (LiCA) was a homogenous method. 7%, respectively. The results suggest that (i) PA can be excluded if 4-hour PAC IA is less than 171 pmol/L, and (ii) PA is highly likely if the PAC IA is greater than 217 pmol/L by chemiluminescence immunoassay. PubMed Google Scholar Nardiello S, Pizella T, Russo M, Galanti B: Serodiagnosis of typhoid fever by enzyme-linked immunosorbent assay determination of anti-Salmonella typhi lipopolysaccharide antibodies. Published online: 1 February 1995. Journal of Pharmaceutical and Biomedical Analysis. 00941-20. The development of novel CLIA with high sensitivity, specificity and throughput has recently become a research focus and trend. Abstract Favorite Journal of Clinical Laboratory Analysis publishes papers on technology & methods for clinical laboratory tests & assays, from molecular diagnostics to genetic testing. The Architect platform requires a minimum of 100 μl of serum or plasma. , 1998). 35, No. 2022;14:67. Methods An in vitro enzyme immunoassay was established with monoclonal antibody PAM4 as the capture reagent, and a Journal of Clinical Laboratory Analysis. The higher sensitivity seen with QL compared to DIR will ensure that fewer RSV infections are missed. Despite impressive technical advances, “mass production” of these assays in a routine Journal of Clinical Microbiology. (SRA or an aggregation assay) together with clinical profiles consistent with a true HIT. The Immulite TSI assay is a chemiluminescence immunoassay with the clinical decision point of 0. Editor The Journal of Clinical Endocrinology & Metabolism, Volume 25, Issue 10, 1 October 1965, Pages 1375–1384, https://doi. Pavelic ZP, Petrelli NJ, Herrera L, et al. All procedures were carried out according to manufacturer's instruction. Journal homepage. The test results showed that the DR-70™ immunoassay kit was capable of detecting cancers with high This study demonstrates a novel approach toward development of advanced immunosensors based on chemically functionalized core−shell Fe3O4@Ag magnetic nanoparticles, and the preparation, characterization, and measurement of relevant properties of the immunosensor useful for the detection of carcinoembryonic antigen (CEA) in clinical Background Among plasma biomarkers for Alzheimer’s disease (AD), pTau181 and pTau217 are the most promising. The diagnostic performance of the (1-3)-β-d-glucan assay for invasive fungal infections has been evaluated in several meta-analyses (20 – 22), and this assay is considered a useful diagnostic tool if it is used for diagnosis among A higher diagnostic cutoff for SSST should be employed when PAC is measured by immunoassay rather than HPLC-MS/MS. Prolactin, a 199 amino acid protein produced by lactotrophs of the anterior pituitary, promotes growth and development of mammary glands and milk production []. Journal of Clinical Virology Plus 3 (2023) 100136 version [9]. Journal of Clinical Laboratory Analysis publishes papers on technology & methods for clinical laboratory tests & assays, Comparison and evaluation of Abbott chemiluminescent microparticle immunoassay and ChIVD light-initiated chemiluminescent assay in the detection of Treponema pallidum antibody. The lower detection limits of the assay for Gram-positive bacteria showed a good agreement with the manufacturer's specifications (8. Hyperprolactinemia is the most frequently encountered endocrine disorder caused by hypothalamic–pituitary axis dysfunction []. Submit an article Journal homepage. Seraj, Harry R. [Google Scholar] BAIRD CW, BORSTEIN J. Immunoassay for tumor markers revealed serum CA 125 of 522. This new assay is designed to achieve improved serocon- Journal of Immunoassay and Immunochemistry Latest Articles. Journal of Cancer Research and Clinical Oncology ll6(1 The inverse trend between sensitivity and assay time should be mentioned, since the fastest assay was presented by Choi et al. Diagnosis may be facilitated by a test for galactomannan antigen detection using an enzyme immunoassay. 17OHP is usually measured by immunoassay but its detection by mass spectrometry (MS) is a potentially superior method. Research Article. The new QL lateral flow assay appears to be a robust assay, showing good sensitivity and specificity in the setting of a busy clinical virology laboratory. Assay of insulin-like activity in the plasma of normal and diabetic human subjects. People also read lists articles that other readers of this article have read. This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4. Methods: This study examined differences in albumin estimation by CZE, Abbott Aeroset BCG, and Aeroset Indian Journal of Clinical Biochemistry - Varying reports across different laboratories or across different analysers in the same lab for the same sample is not an uncommon phenomena. 9. The competing monoclonal antibody used in this assay is specific for a common epitope of The increasing incidence and mortality of infectious diseases caused by carbapenem-resistant Enterobacterales (CRE) have become a global public health threat (1–3). Vol. , 2017; Dipalo et al. However, transition from research to routine clinical use will require confirmation of clinical performance in prospective cohorts and evaluation of cofounding factors. Original Article. The WHO second international standard (IS) for thyroid-stimulating antibodies, NIBSC code 08/204. We also compared To establish a dual immunoassay based on inductively coupled plasma mass spectrometry (ICP-MS) with stable element labeling antibodies for the simultaneous detection The Journal of Immunoassay & Immunochemistry is an international forum for rapid dissemination of research results and methodologies dealing with all aspects of immunoassay and immunochemistry, as well as selected aspects The Journal of Clinical Investigation; Current issue; Past issues; Specialties; Reviews; Review series; Videos; Conversations with Giants in Medicine; Video Abstracts; Collections; IMMUNOASSAY OF ENDOGENOUS PLASMA INSULIN IN MAN Rosalyn S. 04 U/ml, CEA at We compared the clinical performance of the PapilloCheck human papillomavirus (HPV) assay with that of the GP5+/6+-PCR method with an enzyme immunoassay readout (GP5+/6+-PCR-EIA) for the detection of high-risk HPV (hrHPV) types by the use of cervical samples originating from women in a population-based by the use of cervical screening cohort Enzyme immunoassay (EIA) and enzyme-linked immunosorbent assay (ELISA) are both widely used as diagnostic tools in medicine and as quality control measures in various industries; they are also used as analytical tools in Hyperprolactinemia is the most frequently encountered endocrine disorder caused by hypothalamic–pituitary axis dysfunction []. Rose, Troy D. 338. and was equal to 10 min using the SERS-based microdroplet platform, whereas the median LOD for assay in solution was found to be lower than for previously mentioned types of assays . Journal of Clinical Immunology is an international journal that focuses on human immunology, particularly primary immunodeficiencies and related diseases. Background. They have, however, a questionable specificity, especially at lower E2 concentrations, making this method unreliable in postmenopausal women and men (1– 3). A previous study showed that the human SAA turbidimetric immunoassay (hSAA-TIA) could be used to measure the SAA concentration in cats. 19 Results of competitive immunoassays may be falsely elevated by a high biotin concentration in the patient’s serum while results The Journal of Applied Laboratory Medicine, Volume 5, Issue 5, September 2020, Pages 943–953, https://doi Han, Gordana Olbina, Keith Westerman, Elizabeta Nemeth, Tomas Ganz, Karen Copeland, Mark Westerman, Vaughn Ostland, Clinical Immunoassay for Human Hepcidin Predicts Iron Deficiency in First-Time Blood Donors, The Journal of ABSTRACT The diagnostic gains of repeat testing for Clostridium difficile by enzyme immunoassay and PCR (i. Hill, Analysis of Proinflammatory and Anti-Inflammatory Cytokine Serum Concentrations in Patients With Multiple Sclerosis by Using a Multiplexed Immunoassay, American Journal of Clinical Pathology, Volume 136, Issue 5, November 2011 Journal of Clinical Laboratory Analysis publishes papers on technology & methods for clinical The sandwich immunoassay is also considered to be theoretically less susceptible to biotin interference. Volume 58 • Number 8 • 23 July 2020. 1016/S0022-1759(99)00023-X Corpus ID: 29184812; Technical and clinical comparison of two fully automated methods for the immunoassay of CA 125 in serum. Abstract A highly sensitive and reliable Hepatitis B virus surface antigen (HBsAg) measurement is essential to universal screening, timely diagnosis, and management of Hepatitis B the performance of Alinity i, which is a newly developed immunoassay platform, under routine clinical laboratory conditions and to compare the results of Alinity i with those of ARCHITECT i2000SR system. Cite. HBV-DNA tests, such as the branched-chain DNA (b-DNA) signal amplification assay (7, 31), and transcription-mediated amplification (TMA)-based or PCR-based (12, 14, 20) assays are used to diagnose and monitor the efficacy of treatment. The assay is a chemiluminescent microparticle immunoassay for qualitative detection of IgG in human serum or plasma against the SARS-CoV-2 nucleoprotein. 0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as Since the late 19th century, clinical microbiology has evolved alongside our basic understanding of infectious diseases (). 44 Views 0 We meant to explore the association of circRNAs/microRNAs in psoriasis and inspect their correlation with the clinical Scandinavian Journal of Clinical and Laboratory Investigation Volume 84, 2024 - Issue 6. Tumors were classified as responsive (60-100% cell kill), intermediate (30-60% cell kill), and non-responsive (0-30% cell kill) to chemotherapy. Journal of Clinical Microbiology 1983, 18: 872–876. The test uses a 96-well microtiter Journal of Clinical Laboratory Analysis publishes papers on technology & methods for clinical laboratory tests & assays, from molecular diagnostics to genetic testing. Performance evaluation of a rapid dengue NS1 antigen lateral flow immunoassay test with reference to dengue NS1 antigen-capture ELISA. The Amino groups were introduced into Listeria monocytogenes teichoic acids by reductive amination, and the product was coupled to biotin. 021% (CK19), respectively. 1128/jcm. Copy. The objectives of the present study were to assess use of hSAA-TIA for determining the feline SAA concentration and to The procedure is illustrated with data from a virologic quantitative micrococulture assay used to quantify free human immunodeficiency virus in clinical trials. VICTOR HERBERT, KAM-SENG LAU, CHESTER W. pallidum particle agglutination (TPPA). 7 U/L, total beta hCG values of 206. doi: 10. Journal of Clinical Microbiology 1984, 20: 718–721. Accumulating evidence demonstrates that N-terminal Three strains of mice were immunized with Candida tropicalis cell walls, and antibodies against mannan were detected by indirect enzyme immunoassay (EIA) in 3 of 9 BALB/c mice, 4 of 11 C57BL/6 mice, and 4 of 8 CFW mice. Publishes high impact papers The Journal of Immunoassay & Immunochemistry is an international forum for rapid dissemination of research results and methodologies dealing with all aspects of immunoassay and immunochemistry, as well as selected The Journal of Immunoassay & Immunochemistry is an international forum for rapid dissemination of research results and methodologies dealing with all aspects of immunoassay and Explore the current issue of Journal of Immunoassay and Immunochemistry, Volume 46, Issue 1, 2025 Immunoassays have been widely used in many important areas of pharmaceutical analysis such as diagnosis of diseases, therapeutic drug monitoring, clinical pharmacokinetic and The Journal of Immunoassay & Immunochemistry is an international forum for rapid dissemination of research results and methodologies dealing with all aspects of immunoassay and immunochemistry, as well as selected aspects Phase II and III clinical and development batches of Juvista were assayed for content by an immunoenzymometric assay (IEMA) using a National Institute for Biological Standards and Monoclonal antibody based immunoassays have shown that cTnI is undetectable in sera of healthy blood donors. Submissions on clinical studies, thematic reviews and case reports are particularly welcomed. eLocator: 10. A gray zone exists between the cutoffs of 171 and 217 Background: Although a diagnosis of infectious diseases is essential for timely treatment, the performance of diagnostic tests has been hardly evaluated due to variable results that are influenced by multiple factors in different conditions. Hill, Analysis of Proinflammatory and Anti-Inflammatory Cytokine Serum Concentrations in Patients With Multiple Sclerosis by Using a Multiplexed Immunoassay, American Journal of Clinical Pathology, Volume 136, Issue 5, November 2011 Journal of Clinical Laboratory Analysis publishes papers on technology & methods for clinical laboratory tests & assays, from molecular diagnostics to genetic testing. @article{Yan1999TechnicalAC, title={Technical and clinical comparison of two fully automated methods for the immunoassay of CA 125 in serum. 4 million people fell ill with TB and 1. 21 Barthélemy NR, Li Y , Joseph- Mathurin N, et al . Abstract Specific dry reagent immunoassay strip tests for therapeutic drugs have been developed with the apoenzyme reactivation immunoassay system (ARIS). Yalow and Solomon A. Therefore, establishing a highly sensitive time-resolved fluorescence immunoassay for copeptin (copeptin-TRFIA) is helpful to measure stroke and evaluate its value in clinical applications. A neutralization enzyme immunoassay (N-EIA) was developed for the detection of antibody titer rises in sera of patients infected with influenza A (H3N2) virus. 05% VAF for indels and SNVs respectively). Histopathology PFEIFFER EF, PFEIFFER M, DITSCHUNEIT H, AHN CS. which usually are not tested for by clinical laboratories. Assays based on mass spectrometry (MS) represent 60 Validation of a Novel High-Throughput Immunoassay for Measuring Symmetric Dimethylarginine as a Marker of Kidney Function Parker Wilson, Parker Wilson Yale University School of Medicine, New Haven, CT. 09 ng/ml, CA 19. Founded in 1964, the American Society of Clinical Oncology, Inc. Currently, cases of possible syphilis are commonly investigated using the treponemal serological tests T. Paired stool samples obtained within a ten-day period from 366 patients with persistent diarrhea were examined by microscopy. 04% and 0. RESEARCH ARTICLE. , initial negative result followed by positive result) within a 7-day period were 1. Advertisement. Clinical application of Chemiluminescence Microparticle Immunoassay for SARS-CoV-2 infection diagnosis. pallidum IgG Journal of Clinical Laboratory Analysis publishes papers on technology & methods for clinical laboratory tests & assays, from molecular diagnostics to genetic testing. We evaluated the precision, linearity, correlation, and carryover of 16 analytes (HAV Ab IgG, HBsAg The substitution of enzyme immunoassay (EIA) techniques for microscopy as a screening tool forGiardia lamblia infection was assessed. Journal of Pharmaceutical and Biomedical Analysis xxx (2011) xxx–xxx. 1959 Sep 25; 82:479–495. It publishes articles on epidemiology and clinical investigation. This latex immunoassay procedure uses commercially available The assay demonstrated a linear range of 10–800 ng/ml, the cross-reactivity rate at 0. PubMed: 7714201. One advantage of the Liat Journal of Clinical Laboratory Analysis publishes papers on technology & methods for clinical laboratory tests & assays, from molecular diagnostics to genetic testing. Fast Editorial Execution and Review Process is a special service for the article that enables it to get a faster Régis Bouquié, Matthieu Grégoire, Hélène Hernando, Claudine Azoulay, Eric Dailly, Catherine Monteil-Ganière, Alain Pineau, Guillaume Deslandes, Pascale Jolliet, Evaluation of a Methotrexate Chemiluminescent Microparticle Immunoassay: Comparison to Fluorescence Polarization Immunoassay and Liquid Chromatography–Tandem Mass Spectrometry, 60 Validation of a Novel High-Throughput Immunoassay for Measuring Symmetric Dimethylarginine as a Marker of Kidney Function Parker Wilson, Parker Wilson Yale University School of Medicine, New Haven, CT. 14 Testing results were expressed as signal to cutoff (S/CO), and S/CO >1. Berson Radioisotope Service, Veterans Administration Hospital, New York, N. We aimed to investigate the accuracy and clinical performance of PETIA for FC. This study demonstrates a novel approach toward development of advanced immunosensors based on chemically functionalized core−shell Fe3O4@Ag magnetic nanoparticles, and the preparation, characterization, and measurement of relevant properties of the immunosensor useful for the detection of carcinoembryonic antigen (CEA) in clinical Heart failure (HF) is a clinical hallmark of cardiovascular syndrome leading to substantial morbidity, repeated hospitalization, and enormous healthcare expenditure. 25(2):72-74, Jul-Dec 2023. With the characteristics of a short determination time and high specificity, the chemiluminescent immunoassay (CLIA) has been increasingly used in routine clinical applications Syphilis is a multisystem infection caused by the spirochete Treponema pallidum. Editor: Brad Fenwick, University of Tennessee at Knoxville. Assays based on mass spectrometry (MS) represent Serological immunoassays designed to detect specific antibody present in patient samples provide a rapid and sensitive method to monitor for infectious agents, allergy, and autoimmunity (). Alexandria White, Dr. Journal of Clinical Laboratory Analysis publishes papers on technology & methods for clinical laboratory tests Next, the immunoassay strip was removed and placed on a flat horizontal desktop. Alzheimers Res Ther. The evaluation results of the Liat HIV quantitative plasma assay compared to the reference Roche CAP CTM v2. 018% (AFP), 0. In the present study, the performance of the Alinity i system, which is a newly developed immunoassay to diagnose infectious diseases, was evaluated. This test was evaluated using single urine samples collected from 349 patients with lower respiratory tract infection of known A collaborative multicenter study was conducted to evaluate the sensitivity, specificity, and precision of a three-step, fully automated, qualitative microparticle-based enzyme-linked immunoassay (AxSYM HIV Ag/Ab Combo; Abbott Laboratories), designed to simultaneously detect (i) antibodies against human immunodeficiency virus type 1 (HIV-1) Samples were analyzed using pAb immunoassay (Abbott Architect Cortisol), mAb immunoassay (Roche Elecsys Cortisol II), and mass spectrometry (MS). The Journal of Applied Laboratory Medicine, Volume 5, Issue 5, September 2020, Pages 943–953, https://doi Han, Gordana Olbina, Keith Westerman, Elizabeta Nemeth, Tomas Ganz, Karen Copeland, Mark Westerman, Vaughn Ostland, Clinical Immunoassay for Human Hepcidin Predicts Iron Deficiency in First-Time Blood Donors, The Journal of Comparison of Two Commercially Available Fentanyl Screening Immunoassays for Clinical Use Melissa M Budelier, Melissa M Budelier Department of Pathology and Immunology, Division of Laboratory and Genomic Medicine, Washington University The Journal of Applied Laboratory Medicine, Volume 5, Issue 6, November 2020, Pages 1277–1286, https An Overview on Interference in Clinical Immunoassays A Cause for Concern. The Bartels Legionella Urinary Antigen enzyme immunoassay (Intracel, USA) is intended for the presumptive diagnosis of past or current Legionnaires' disease by qualitative detection of Legionella pneumophila serogroup 1 antigen in human urine. Volume 7, Issue 2 p. Volume 35, Issue 12 e23950. In this assay, the p30 protein of ASFV was genetically coupled to the LgBiT and SmBiT subunits of nanoluciferase, which were used as fusion probes for specific antibodies. 74 Views 0 Particle-enhanced turbidimetric immunoassay (PETIA) is a new measurement procedure for detecting fecal calprotectin (FC). The minimum detectable concentration was 0. Immunoassay Measurements After Plasma Purification. We aimed to assess the clinical diagnostic performance of LiCA and compare it with that of chemiluminescence immunoassay (CLIA) which was widely used in clinical laboratories. Some of the most commonly used methods in clinical laboratories are enzyme-linked immunosorbent assay (ELISA), time-resolved fluorescence immunoassay (TRFIA), chemiluminescence Journal of Clinical Laboratory Analysis. istics of a given assay; whereas, clinical qualification is the evidentiary and statistical. Cited by lists all citing articles based on Crossref citations. As we appreciate now more than ever, the relationship Journal of Immunoassay and Immunochemistry 2024-2025 Journal's Impact IF is 0. There is little value of repeat Journal of Clinical Virology. Its expression is under constant suppression by dopamine but plasma Journal of Clinical Microbiology Vol. It has not, however, been shown to produce a clinical false-negative result (Kunsman et al. PDF (116K) Actions. Jarvis et al. org Supplementary Data; Cite. Delayed diagnosis and therapy may lead to poor outcomes. Use of immunoassays and other ligand-binding assays in clinical diagnosis has increased dramatically during the last several years. Y. 1 ng/mL in sera of 95% of hospitalized patients First, we compared the limit of detection (LOD) as a key performance parameter for 30 fluorescence and 30 SERS-based immunoassays reported in the literature. 105-110. American Journal of Clinical Pathology, Volume 149, Issue suppl_1, January 2018, Journal of Clinical Laboratory Analysis publishes papers on technology & methods for clinical laboratory tests & assays, from molecular diagnostics to genetic testing. g. A serum calprotectin immunoassay was developed Journal of Clinical Chemistry and Laboratory Medicine is participating in the Fast Editorial Execution and Review Process (FEE-Review Process) with an additional prepayment of $99 apart from the regular article processing fee. Compared with the Roche platform, the clinical sensitivity of the Liat assay at a cutoff of 1,000 copies/ml was 100%, and the specificity was 88. As elderly populations continue to increase, biomarker-guided diagnosis and treatment for age-related cardiac decline have become essential. 01626-17. does a low concentration of biotin cause interference), (2) the prevalence of biotin at Thomas B. History. 8 Immunoassay-based techniques are routinely used in clinical and research settings for the measurement of serum estradiol (E2) levels. The assay was also tested for cross-reactivity against 19 mecA-negative non-S. There were no interferences from plasminogen Journal of Medical Virology is a clinical virology journal focused on the diagnosis, epidemiology, immunology and pathogenesis of human viral infections & diseases. aureus isolates. Abstract Background Reports of false laboratory Journal of Clinical Immunology is an international journal that focuses on human immunology, particularly primary immunodeficiencies and related The serum biomarker copeptin, an innovative and stable substitute biomarker of vasopressin, is associated with stroke. Compared with Over the last 2 decades, nucleic acid detection by PCR has emerged as a promising tool for early diagnosis of IA (6, 11, 20). Assessment of efficacy of dendritic cell therapy and viral therapy in high grade glioma clinical trials. In addition, two commercially availableGiardia lamblia-specific ElAs were performed. Aim of this study was to compare the new highly sensitive Liaison Tg II (Tg-L) with the well-established Tg Access assay (Tg-A). (ASCO®) is committed to making a world of difference in W. Ann N Y Acad Sci. 0 and Abbott RealTime HIV-1 assays showed good performance. Double antibody sandwich was used to establish Immunoassay-based techniques are routinely used in clinical and research settings for the measurement of serum estradiol (E2) levels. Volume 3, Issue 2, June 2023, 100144. GOTTLIEB, SHELDON J. 05 ng/ml; the intraassay coefficient of variation (CV) and interassay CV were both less than 10%, with good stability and reproducibility. 2019 ; Development of a Clinical Chemiluminescent Immunoassay for Serum GPC3 and Simultaneous Measurements Alone With AFP and CK19 in Diagnosis of Hepatocellular Carcinoma. China. Tuberculosis (TB) is the number one cause of death due to infectious disease. Although the international clinical chemistry community tries to standardize or harmonize methods (61, 66, 67), this is not an easy job. Wilson, Dee Husebye, Hanieh S. / Analytical and clinical performance of newly developed immunoassay for detecting thyroid-stimulating immunoglobulin, the Immulite TSI assay. This study shows that hemolysis can negatively interfere with C-peptide assay in clinical laboratory practice, and this effect increased with the degree and exposure time of Journal of Clinical Immunoassay 7: 125; 1984. Paula Jenkins Colon, Dina N Greene, Biotin Interference in Clinical Immunoassays, The Journal of Applied Laboratory Medicine, Volume 2, Issue 6, 1 May 2018, Pages 941–951, Clinical assay manufacturers are required by the FDA to Syphilis is a multisystem infection caused by the spirochete Treponema pallidum. It was concluded that biomarker assays must be validated in a manner that provides reliable and accurate data as basis A PCR assay for bla NDM was conducted with primers NDM-1_a_fw (5′-CAATATTATGCACCCGGTCG-3′) and NDM-1_a_rev Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM) Other Formats. Immunoassays have been widely used in many important areas of pharmaceutical analysis such as diagnosis of diseases, therapeutic drug monitoring, clinical Many hepatitis B virus (HBV) markers are used for diagnosing and monitoring hepatitis B patients. Anna Wu. 0%, 91. They include receptor assay, enzyme-linked immunosorbent assay (ELISA) in all of its embodiments, ligand-based assays, Development and evaluation of a 16S ribosomal RNA-based polymerase chain reaction followed by Sanger sequencing assay for the identification of bacterial pathogens: Three years' experience from a clinical microbiology laboratory in Eastern India Journal of The Academy of Clinical Microbiologists. Volume 33 • Number 2 • February 1995. To validate the CPT-strip assay, 324 clinical isolates of S. 55 IU/L provided by the manufacturer. Despite a variety of “in-house” specific primers and conditions reported in published studies (32, 42), the lack of clinical validation of the laboratory-developed assays has prevented the inclusion of Aspergillus PCR in the European Organization for Research and We have evaluated a new enzyme immunoassay technology to detect the products of PCR-based amplification that may be applicable to routine testing of hepatitis B virus (HBV) DNA. Vischio, MD , Invasive aspergillosis is a serious and often fatal infection in patients who are neutropenic or have undergone solid organ or stem cell transplantation. Xiaohui Chen, Xiaohui Chen. ABSTRACT Background Detection of IgG subclasses (IgGSc) is vital for the diagnosis and management of disease, especially IgG4-related diseases (IgG4-RD). Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine. Methods. 5 × 10 4 . 7 million died from the disease (). 06 m IU/ml, AFP at 4. TB is also the most common overall cause of mortality in people living with human immunodeficiency virus (HIV), with an estimated 374,000 deaths in 2016 (). We evaluated the precision, linearity, correlation, and carryover of 16 Journal of Clinical Laboratory Analysis publishes papers on technology & methods for clinical laboratory tests & assays, from molecular diagnostics to genetic testing. Double antibody sandwich was used to establish DOI: 10. However, these products contain riboflavin and creatine, which aid in avoiding detection of dilution. Evaluation of Fluorescence Microsphere Immunoassay for Detection of Antibodies to Rift Valley Fever Virus Nucleocapsid Protein and Glycoproteins. In the present study, the performance of the Alinity i system, which is a newly developed immunoassay to diagnose infectious The Journal of Immunoassay & Immunochemistry is an international forum for rapid dissemination of research results and methodologies dealing with all aspects of immunoassay and immunochemistry, as well as selected aspects of immunology. 3 A general primer GP5+/GP6(+)-mediated PCR-enzyme immunoassay method for rapid detection of 14 high-risk and 6 low-risk human papillomavirus genotypes in cervical scrapings The competitive enzyme immunoassay (CELISA) for the detection of serum antibody to Brucella is a multispecies assay which appears to be capable of differentiating vaccinal and cross-reacting antibodies from antibodies elicited by field infection in cattle. In China, more than 70% of the CRE strains is carbapenem-resistant Klebsiella pneumoniae (CRKP), and the mortality rate of infections caused by CRKP strains in hospitalized patients Thomas B. 020% (carcino-embryonic antigen), and 0. A Kim, Jin Ju ; Jeong, Soon Ho ; Kim, Byungkwang et al. . As a library, NLM provides access to scientific literature. Journal of Clinical Microbiology Vol. Responding mice produced Journal of Clinical Oncology. 0% (310/313) and an overall sensitivity of 80. About ASCO. We demonstrate the clinical utility of iTACT-HBcrAg for monitoring chronic hepatitis B (CHB) and for the early detection of HBV reactivation. All the 94 positive immunoassay patients were tested with an aggregation assay, except for 3 patients (96. BRIEF REPORT. These disciplines trace their roots to the work of Pasteur, Koch, and Lister, from whom Germ Theory introduced the revolutionary concept that particular microbes are responsible for specific infections (). (0. 1186/s13195-022-01005-8. Author links open overlay panel Mohammed Rashiku a, Kapil Manoharan a, Nitiksha Rani b, Jasmine Samal b, Ekta Gupta b, Shantanu Immunoassays are bioanalytical methods in which the quantitation of the analyte depends on the reaction of an antigen (analyte) and an antibody. does a low concentration of biotin cause interference), (2) the prevalence of biotin at that concentration and (3) the consequences of a change in the result, which depends on the analyte. A world where cancer is prevented or cured. e. BLEICHER, Coated Charcoal Immunoassay of Insulin, The Journal of Clinical Endocrinology & Metabolism, Volume 25, Issue 10, 1 October 1965, The use of such dextran-coated charcoal makes simpler and more rapid the immunoassay of insulin in biologic fluids, periodical Journal of clinical immunoassay Published in New York NY) by Kellner-McCaffery Print available from 1983-1994 However, the results of the magnetic bead fluorescent immunoassay (MB-FIA) and the fluorescence immunochromatography assay (FICA) were not accurate enough [11, 12]. We describe the first CLIA-validated immunoassay with excellent accuracy and precision to quantify human serum hepcidin. Hepcidin, a key regulator of iron homeostasis, is reduced in iron deficiency (ID) and increased in iron overload. Martins, John W. Simpson, L. , 2019). Alzheimers Res Ther 2022;14:67. Riboflavin adds color to urine and, thus, helps to produce a realistic-looking sample; in addition, it interferes with the FPIA immunoassay. Sequeira, Shiefa. AmniSure Placental Alpha Microglobulin-1 Rapid Immunoassay for Detection of Rupture of Membranes: A Comparison Study With Clinical-Based Testing Get access Anna T. Jaskowski, Andrew R. To establish a dual immunoassay based on inductively coupled plasma mass spectrometry (ICP-MS) with stable element labeling antibodies for the simultaneous detection of alpha-fetoprotein (AFP) and prostate-specific antigen (PSA) in serum and evaluate its performance and clinical sample validation. The sensitivity and specificity of the assay are examined and compared to results from a CA19-9 immunoassay. 2% in early-stage, late-stage, and In a blinded study of 11 patients affected by Glioblastoma Grade IV we assessed the accuracy of the ChemoID assay by comparing the test results to clinical response. 9% (233/288), with sensitivities of 64. Immunoassays were repeated in 21 plasma samples from 7 patients with discordant results (in whom there was sufficient remaining banked plasma) after purification of plasma to remove possible interfering macromolecules (eg, heterophile antibodies) as detailed in the supplement . In 2016, 10. Diagnostic immunoassays and kits designed to measure antibodies typically include one or more components containing the specific antibody being measured that The clinical significance of biotin interference depends on three factors: (1) the susceptibility of an immunoassay to biotin interference (e. Christopher P Price, The Evolution of Immunoassay as Seen Through the Journal Clinical Chemistry, Clinical Chemistry, Volume 44, Issue 10, 1 October 1998, The Evolution of Immunoassay as Seen Through the Journal Clinical Chemistry - 24 The clinical significance of biotin interference depends on three factors: (1) the susceptibility of an immunoassay to biotin interference (e. A total of 10 772 patients from the Peking University Third Hospital were enrolled. In addition, we tested the effect of growing a subset of the clinical isolates on three commonly used culture media prior to testing by the CPT Chemiluminescence immunoassay (CLIA) has been widely used in different fields including clinical diagnosis, food safety, and environmental assessment for its high sensitivity, selectivity, and rapid and simple analysis. In: Scandinavian Journal of Clinical and Laboratory Investigation. In clinical validation, the assay demonstrated a specificity of 99. Author links open overlay panel Wanbing Liu a 1, Guomei Kou a 1, Yaoyu Dong b, Yaqiong Zheng a, Yinjuan Ding a, Wenxu Ni a, Wanlei Wu a, Shi Tang a, Zhou Xiong a, Ying Journal of Clinical Oncology Podcast Jan 09, 2025. Volume 55, Issue 5, 15 July 2011, Pages 869-877. VIEW ALL PODCASTS. The assay employs a multiplex PCR using 14 primer pairs, followed by a multiplex TSPE reaction using 21 primer pairs, to detect and identify 20 different virus types and subtypes in a single test. Tsoi, C. In this N-EIA, a selected strain of influenza A (H3N2) virus was added to monolayers of LLC-MK2 Journal List User Guide PERMALINK. Early View. In addition, the correlation between the results of LICA and capture enzyme-linked immunosorbent assay was evaluated. aureus were tested by the CPT-strip test. 3 A general primer GP5+/GP6(+)-mediated PCR-enzyme immunoassay method for rapid detection of 14 high-risk and 6 low-risk human papillomavirus genotypes in cervical scrapings When the immunoassay results do not appear to fit the clinical picture, it becomes a great challenge to the clinical biochemist, alarms clinicians and confounds immunoassay manufacturers. AI was diagnosed if serum cortisol level was <18 using the pAb immunoassay. Articles with the Crossref icon will open in a new tab. 2% . Abstract Background Hepatitis C virus antibody (anti-HCV) test had been approved as a preliminary screening test for HCV infection. The assay performance was confirmed per the clinical guideline documents. R. Journal of Clinical Laboratory Analysis. Teichoic acids were assayed by their binding to specific antibody adsorbed to a solid phase, followed by detection of the antigen-antibody complex by horseradish peroxidase-avidin. Pages: 413 - 415. Background Various factors may affect the accuracy of hemoglobin (Hb) A1c measurements that are widely used to monitor glycemic control in diabetic patients. cTnI concentration is <3. The AAPS and Clinical Ligand Assay Society cosponsored a Biomarker Method Validation Workshop in October 2003 purpose [13]. In this context, the performance of new-generation thyroglobulin assay has clinical implications in the follow-up of DTC patients. Volume 56 • Number 6 • June 2018. The nontreponemal rapid plasma reagin (RPR) flocculation test Journal of Thrombosis and Thrombolysis - Heparin induced thrombocytopenia (HIT) is a life and limb-threatening complication of heparin exposure. 63 Views 13 A clinical study using DR-70™ immunoassay for the detection of 13 different cancers have been conducted with 277 healthy subjects and 136 cancer patients. D-14 monoclonal antibody to carcinoembryonic antigen: immunohistochemical analysis of formalin-fixed, paraffin-embedded human colorectal carcinoma, tumors of non-colorectal origin and normal tissues. Journal of Clinical Microbiology. The ARCHITECT Syphilis TP assay is a two‐step immunoassay for qualitative detection of antibody to TP in human serum or plasma using CMIA technology with assay protocols, referred to as Chemiflex. BLEICHER, Coated Charcoal Immunoassay of Insulin, The Journal of Clinical Endocrinology & Metabolism, Volume 25, Issue 10, 1 Busolo F, Tonin E, Conventi L: Enzyme-linked immunosorbent assay for detection ofMycoplasma pneumoniae antibodies. When method changes or assay updates occur during the course of a study, provisional solutions have to be found to deal with these changes for the current studies. PubMed Google Scholar . Davide Soldato, Dr. Journal of Clinical Virology Plus. Journal of Immunoassay Volume 19, 1998 - Issue 1. Journal of Clinical Laboratory Analysis publishes papers on technology & methods for clinical laboratory tests & assays, The CLIA and the Siemens immunonephelometric assay appeared to have good consistency with comparable diagnostic value in detecting IgGSc, especially IgG4, and IgG1 that can accurately identify IgG4-RD or pSS in clinical 17α‐hydroxyprogesterone (17OHP) is the most important serum marker for congenital adrenal hyperplasia (CAH). Measurements by MS and mAb immunoassay were individually used in simple logistic regression models to predict AI. Abstract. Other rapid methods for diagnosis include Background/Aims: The introduction of capillary zone electrophoresis (CZE) to this laboratory has highlighted discrepancies in albumin measured on an Abbott Aeroset by bromocresol green (BCG) and that calculated by CZE on the basis of total protein measured by Biuret. Application of the PGD immunoassay showed that it is a rapid test with a short hands-on time for sample processing and no demand for special technical equipment and instrument operation. Journal of Clinical Microbiology 1980, 12: 69–73. Clinical and experimental studies of insulin secretion following tolbutamide and metahexamide administration. However, these Immunoassay of Insulin-Like Growth Factor-Binding Protein-3 (IGFBP-3): New Means to Quantifying IGFBP-3 Proteolysis New Means to Quantifying IGFBP-3 Proteolysis, The Journal of Clinical Endocrinology & Metabolism, Volume 85, Issue 6, 1 Journal of Clinical Tuberculosis and Other Mycobacterial Diseases aims to provide a forum for clinically relevant articles on all aspects of tuberculosis and other mycobacterial infections. 2 × 10 3 to 5. Volume 42, Number 16_suppl June 2024. Volume 130, September 2020, 104576. 7%, and 85. Its expression is under constant suppression by dopamine but plasma Journal of Clinical Laboratory Analysis publishes papers on technology & methods for clinical laboratory tests & assays, As compared with the previous immune complex transfer enzyme immunoassay, the procedure was simplified by reducing one incubation step and one washing step, and the detection limit of antithyroglobulin IgG in serum (0. The collected swab sample from the deep pharynx, meat, or feces was then placed into a sample diluent with stirring five times and immersed for 1 min. 45, No. The evaluation was conducted in accordance with objective recommen-dations for analytical performance (Clinical and Laboratory Standards Journal of Clinical Laboratory Analysis publishes papers on technology & methods for clinical laboratory In the present study, the performance of the Alinity i system, which is a newly developed immunoassay to diagnose infectious diseases, was evaluated. Volume 35, Issue 2 e23589. Abstract We describe a rapid and sensitive latex nephelometric immunoassay for quantifying ferritin in human serum. Most of the deaths from TB could have been The serum biomarker copeptin, an innovative and stable substitute biomarker of vasopressin, is associated with stroke. Automated latex nephelometric immunoassay for the measurement of serum lipoprotein (a) This assay generates a standard curve in the range of 27 to 1750 mg/L, showing inter-assay precision of less than 8%. This simple and Journal of Clinical Laboratory Analysis publishes papers on technology & methods for clinical laboratory tests & assays, from molecular diagnostics to genetic testing. Cite; Collections. 1 As the demand for diagnostic tests involving PCT assessment is increasing rapidly, many methods of measuring PCT have emerged (Ceriotti et al. J Endocrinol. Method pTau181 and pTau217 were quantified using, Quanterix and ALZpath, SIMOA assays Serum markers currently used as indicators of iron status have clinical limitations. Dussaix E, Slim A, Tournier P: Comparison of enzyme-linked immunosorbent assay and complement fixation test for detection ofMycoplasma pneumoniae antibodies. -C. The test results showed that the DR-70™ immunoassay kit was capable of detecting cancers with high Alzheimer’s disease and clinical progression of a novel assay for plasma P- Tau217. hmvmq chb pzc qepmes yjezq hgjki cemu fapdsjz qhra ktnt